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Literature summary extracted from

  • Yoshimura, N.; Kikuchi, T.; Sasaki, T.; Kithara, A.; Hatanaka, M.; Murachi, T.
    Two distinct Ca2+ proteases (calpain I and calpain II) purified concurrently by the same method from rat kidney (1983), J. Biol. Chem., 258, 8883-8889.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.52 Ba2+ activates Rattus norvegicus
3.4.22.52 Ca2+
-
 Homo sapiens
3.4.22.52 Ca2+ half-maximal activation at 0.002 mM, full activity at 0.01 mM Rattus norvegicus
3.4.22.52 Mn2+ activates Rattus norvegicus
3.4.22.52 Sr2+ activates Rattus norvegicus
3.4.22.53 Ba2+ slight activation Rattus norvegicus
3.4.22.53 Ca2+ half-maximal activation at 0.2 mM, full activity at 1 mM Rattus norvegicus
3.4.22.53 Mg2+ slight activation Rattus norvegicus
3.4.22.53 Sr2+ activates Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.52 25000
-
 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
3.4.22.52 80000
-
 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
3.4.22.52 110000
-
 gel filtration Rattus norvegicus
3.4.22.53 25000
-
 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
3.4.22.53 80000
-
 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
3.4.22.53 115000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.52 Homo sapiens
-
-
-
3.4.22.52 Rattus norvegicus
-
-
-
3.4.22.53 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.52
-
 Rattus norvegicus
3.4.22.53
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.52 additional information
-
-
 Homo sapiens
3.4.22.52 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.52 casein + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.53 casein + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.52 dimer
-
 Homo sapiens
3.4.22.52 dimer 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
3.4.22.53 dimer 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.52 58
-
 10 min, more than 30% of the original activity is left Rattus norvegicus
3.4.22.53 58
-
 10 min, complete inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.53 7.5 8
-
 Rattus norvegicus